Anti-DAG1 Anticorps (A82725)

Name: Anti-DAG1 Antibody
Brand: Antibodies.com
SKU: A82725
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab103905). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre DAG1 pour ELISA, WB, IHC, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-DAG1 Antibody

Description du produit

Goat polyclonal antibody to DAG1.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 0.1-0.3µg/ml, IHC: 5µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human DAG1.

Séquence

C-HVGKHEYFMHATDK

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

100 kDa

MW prédit

97.4kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

156DAG, A3a, Agrin receptor, AGRNR, Alpha-DG, alpha-DG-N, Beta-DG, Beta-dystroglycan, DAG, DAG1_HUMAN, Dystroglycan, Dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystroglycan, alpha, Dystrophin-associated glycoprotein 1, MDDGC7, MDDGC9, OTTHUMP00000210857, OTTHUMP00000210858

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-DAG1 Antibody (A82725)

DAG1 expression in Human Skeletal Muscle lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DAG1 Antibody (A82725) at 0.2µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-DAG1 Antibody (A82725)

DAG1 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-DAG1 Antibody (A82725) at 5µg/ml and revealed with alkaline phosphatase (AP).

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Figure 3: Immunofluorescence - Anti-DAG1 Antibody (A82725)

DAG1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-DAG1 Antibody (A82725) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-DAG1 Antibody (A82725)

DAG1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-DAG1 Antibody (A82725) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.