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Anti-Cytokeratin 5 Anticorps (A14122)

Western Blot - Anti-Cytokeratin 5 Antibody (A14122) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 5 Antibody (A14122) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 5 Antibody (A14122) - Antibodies.com Western Blot - Anti-Cytokeratin 5 Antibody (A14122) - Antibodies.com
$335
Product Datasheet

Anticorps polyclonal de lapin contre Cytokeratin 5 pour WB, IHC, ICC/IF et IP.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-Cytokeratin 5 Antibody
Description du produit
Rabbit polyclonal antibody to Cytokeratin 5.
Applications
WB, IHC, ICC/IF, IP
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:2,000-1:6,000
Reactivité
Human, Mouse
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 5 (KRT5) (NP_000415.2).
Séquence
MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGG
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
62 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
58 kDa cytokeratin, CK-5, CK5, Cytokeratin-5, Cytokeratin5, DDD, DDD1, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K2C5_HUMAN, K5, Keratin, Keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), keratin complex 2, basic, gene 5, keratin, type II cytoskeletal 5, Keratin-5, Keratin5, KRT 5, Krt5, KRT5A, type II cytoskeletal 5, Type-II keratin Kb5
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Cytokeratin 5 Antibody (A14122) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Cytokeratin 5 Antibody (A14122)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 5 Antibody (A14122) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Cytokeratin 5 Antibody (A14122) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-Cytokeratin 5 Antibody (A14122)
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Cytokeratin 5 Antibody (A14122) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-Cytokeratin 5 Antibody (A14122)
Immunofluorescence analysis of L929 cells using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-Cytokeratin 5 Antibody (A14122) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - Anti-Cytokeratin 5 Antibody (A14122)
Immunoprecipitation analysis of 200µg extracts of A-431 cells using 3µg of Anti-Cytokeratin 5 Antibody (A14122). This Western blot was performed on the immunoprecipitate using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:5000.

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