Anti-Cytokeratin 20 Antibody [SPM191] - BSA and Azide free
Description
Mouse monoclonal [SPM191] antibody to Cytokeratin 20.
Specificity
This antibody recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 196-323, of human KRT20 protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
SPM191
Isotype
IgG2b
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
46 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Cytokeratin 20 Antibody [SPM191] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Western Blot - Anti-Cytokeratin 20 Antibody [SPM191] - BSA and Azide free (A252853)
Western blot analysis of human HT29 cell lysate using Anti-Cytokeratin 20 Antibody [SPM191].
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