Anti-Cytokeratin 20 Antibody [KRT20/4879R] - BSA and Azide free
Description
Recombinant rabbit monoclonal [KRT20/4879R] antibody to Cytokeratin 20.
Specificity
This antibody recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Applications
IHC-P
Dilutions
IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 196-323, of human Cytokeratin 20. The exact sequence is proprietary.
Host
Rabbit
Clonality
Monoclonal
Clone ID
KRT20/4879R
Isotype
IgG
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
46 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain using Anti-Cytokeratin 20 Antibody [KRT20/4879R] at 2µg/ml in PBS for 30 minutes at room temperature. Inset: PBS instead of the primary antibody. Secondary antibody negative control.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon using Anti-Cytokeratin 20 Antibody [KRT20/4879R] at 2µg/ml in PBS for 30 minutes at room temperature.
SDS-PAGE analysis of Anti-Cytokeratin 20 Antibody [KRT20/4879R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
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