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Anti-Cytokeratin 20 Anticorps [ARC0288] (A306519)

Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com
$470
Product Datasheet

Anticorps monoclonal [ARC0288] de lapin contre Cytokeratin 20 pour WB et IHC.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-Cytokeratin 20 Antibody [ARC0288]
Description du produit
Rabbit monoclonal [ARC0288] antibody to Cytokeratin 20.
Applications
WB, IHC
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivité
Human, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 325-424 of human Cytokeratin 20 (KRT20) (P35900).
Séquence
LANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0288
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
44 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CD20, CK 20, CK-20, CK20, Cytokeratin-20, Cytokeratin20, K1C20_HUMAN, K20, KA20, Keratin, Keratin 20, keratin 20, type I, keratin 21, rat, homolog of, Keratin type I cytoskeletal 20, Keratin-20, Keratin20, KRT 20, KRT 21, KRT20, KRT21, MGC35423, OTTHUMP00000164518, Protein IT, type I cytoskeletal 20
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Western blot analysis of extracts of HT-29 cells, using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Western blot analysis of extracts of Rat liver, using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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