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Anti-Cytokeratin 19 Anticorps [ARC2811] (A307943)

Nom du produit
Anti-Cytokeratin 19 Antibody [ARC2811]
Description du produit
Rabbit monoclonal [ARC2811] antibody to Cytokeratin 19.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 19 (KRT19) (KRT19) (P08727).
Séquence
MTSYSYRQSSATSSFGGLGGGSVRFGPGVAFRAPSIHGGSGGRGVSVSSARFVSSSSSGAYGGGYGGVLTASDGLLAGNEKLTMQNLNDRLASYLDKVRA
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC2811
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
43 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
40 kDa keratin intermediate filament, CK 19, CK-19, CK19, Cytokeratin-19, K19, K1C19_HUMAN, K1CS, Keratin 19, Keratin type I 40 kD, Keratin type I 40kD, Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, Keratin, type I, 40 kd, Keratin-19, KRT19, MGC15366
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)
Western blot analysis of various lysates, using Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)
Immunohistochemistry analysis of paraffin-embedded human hepatocellular carcinoma (negative control sample) using Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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