Mouse monoclonal [DA-7] antibody to Cytokeratin 18.
Specificity
This antibody reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Applications
IP, WB, IHC-P, ICC, ELISA
Dilutions
WB: 1-2 µg/ml, IHC-P: 10 µg/ml; Positive Control: colon, skin, IHC: Positive Control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line, ELISA: Positive Control: MCF-7 human breast adenocarcinoma cell line; This antibody has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100).
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Dog, Mouse, Rat, Pig, Hamster, Sheep or Cow.
Immunogen
Human breast carcinoma cell line PMC-42.
Host
Mouse
Clonality
Monoclonal
Clone ID
DA-7
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cell proliferation-inducing gene 46 protein, CK-18, CYK18, Cytokeratin-18, K18, Keratin, type I cytoskeletal 18, Keratin-18, KRT18
Western Blot - Anti-Cytokeratin 18 Antibody [DA-7] (A86584)
Western Blotting analysis (reducing conditions) of whole cell lysate using Anti-Cytokeratin 18 Antibody (A86584). Lane 1: HeLa human cervix carcinoma cell line. Lane 2: A-431 human epidermoid carcinoma cell line. Lane 3: MCF-7 human breast adenocarcinoma cell line.
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