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Anti-Cytokeratin 18 Anticorps [ARC2303] (A305288)

Nom du produit
Anti-Cytokeratin 18 Antibody [ARC2303]
Description du produit
Rabbit monoclonal [ARC2303] antibody to Cytokeratin 18.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:1,000-1:3,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 250-430 of human Cytokeratin 18 (KRT18) (P05783).
Séquence
ADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC2303
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
48 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Cell proliferation inducing gene 46 protein, Cell proliferation inducing protein 46, Cell proliferation-inducing gene 46 protein, CK 18, CK-18, CK18, CYK 18, CYK18, Cytokeratin endo B, Cytokeratin-18, K 18, K18, K1C18_HUMAN, KA18, Keratin 18, Keratin 18, type I, Keratin D, keratin, type I cytoskeletal 18, Keratin-18, Krt18
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)
Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)
Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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