Rabbit monoclonal [ARC1559] antibody to Cyclin E1 (phospho Thr77).
Applications
WB
Recommander des dilutions
WB: 1:500-1:2,000
Reactivité
Human, Mouse
Immunogène
A synthetic phosphorylated peptide around T77 of human Cyclin E1 (P24864).
Séquence
IPTPD
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC1559
Isotype
IgG
Conjuguer
Unconjugated
Purification
Affinity purification.
Masse moléculaire
54 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Figure 1: Western Blot - Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326) at 1:1,000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.
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