Rabbit polyclonal antibody to Cyclin E1 (phospho Thr395).
Applications
WB, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic phosphorylated peptide around T395 of human Cyclin E1 (NP_001229.1).
Séquence
LLTPP
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Affinity purification.
Masse moléculaire
55 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Figure 1: Western Blot - Anti-Cyclin E1 (phospho Thr395) Antibody (A10865)
Western blot analysis of extracts of various cell lines, using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunofluorescence analysis of C6 cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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