Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Stockage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Notes générales

This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.

Synonymes

C-X-C chemokine receptor type 4, CD184, CD184 antigen, Chemokine (C X C motif) receptor 4, Chemokine CXC Motif Receptor 4, CXC-R4, CXCR-4, CXCR4_HUMAN, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP 3, LAP3, LCR1, LESTR, Leukocyte derived seven transmembrane domain receptor, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide associated protein 3, Neuropeptide Y receptor Y3, NPY3R, NPYR, NPYRL, NPYY3, NPYY3R, Probable G protein coupled receptor lcr1 homolog, SDF 1 receptor, SDF-1 receptor, SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR, Stromal cell derived factor 1 receptor, Stromal cell-derived factor 1 receptor, WHIM, WHIMS

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Flow Cytometry - Anti-CXCR4 Antibody [DMC680] - BSA and Azide free (A318688)

CXCR4 protein is expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CXCR4 Antibody [DMC680] - BSA and Azide free (A318688) on Expi293 cells transfected with human CXCR4 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

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Figure 2: Flow Cytometry - Anti-CXCR4 Antibody [DMC680] - BSA and Azide free (A318688)

Flow cytometry analysis of antigen binding of Anti-CXCR4 Antibody [DMC680] - BSA and Azide free (A318688). (A) Anti-CXCR4 Antibody [DMC680] - BSA and Azide free (A318688) does not bind to hepG2 cells that do not express CXCR4. (B) A clear peak shift of Anti-CXCR4 Antibody [DMC680] - BSA and Azide free (A318688) was seen compared to the control when incubated with CXCR4-expressing Hela cells, indicating strong binding of Anti-CXCR4 Antibody [DMC680] - BSA and Azide free (A318688) to CXCR4. Antibodies were incubated at 5 µg/ml. .

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