Western Blot - Anti-Crk p38 (phospho Tyr221) Antibody (A89608)
Western blot analysis of extracts of K562 cells, using Anti-Crk p38 (phospho Tyr221) Antibody (A89608) at 1:1,000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.
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