Anti-Creatine Kinase MB Antibody [ARC1990] (A307604)

Name: Anti-Creatine Kinase MB Antibody [ARC1990]
Brand: Antibodies.com
SKU: A307604
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1990] antibody to Creatine Kinase MB for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Creatine Kinase MB Antibody [ARC1990]

Description

Rabbit monoclonal [ARC1990] antibody to Creatine Kinase MB.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Creatine kinase B type (CKB) (P12277).

Sequence

MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1990

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CKM, CKMM, CPK-M, Creatine kinase M chain, Creatine kinase M-type, Creatine phosphokinase M-type, M-CK

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Creatine Kinase MB Antibody [ARC1990] (A307604)

Western blot analysis of extracts of various cell lines, using Anti-Creatine Kinase MB Antibody [ARC1990] (A307604) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-Creatine Kinase MB Antibody [ARC1990] (A307604)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Creatine Kinase MB Antibody [ARC1990] (A307604) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Creatine Kinase MB Antibody [ARC1990] (A307604)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Creatine Kinase MB Antibody [ARC1990] (A307604) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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