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Anti-CRB1 Antibody (A8756)

Western Blot - Anti-CRB1 Antibody (A8756) - Antibodies.com Immunohistochemistry - Anti-CRB1 Antibody (A8756) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to CRB1 for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-CRB1 Antibody
Description
Rabbit polyclonal antibody to CRB1.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 28-133 of human CRB1 (NP_957705.1).
Sequence
NKNNTRCLSNSCQNNSTCKDFSKDNDCSCSDTANNLDKDCDNMKDPCFSNPCQGSATCVNTPGERSFLCKCPPGYSGTICETTIGSCGKNSCQHGGICHQDPIYPV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
150 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-CRB1 Antibody (A8756) - Antibodies.comEnlarge Image

Western Blot - Anti-CRB1 Antibody (A8756)

Western blot analysis of extracts of HeLa cells, using Anti-CRB1 Antibody (A8756) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-CRB1 Antibody (A8756) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-CRB1 Antibody (A8756)

Immunohistochemistry analysis of paraffin-embedded mouse retina using Anti-CRB1 Antibody (A8756) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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