Anti-CRABP1 Antibody [ARC1991] (A308999)

Name: Anti-CRABP1 Antibody [ARC1991]
Brand: Antibodies.com
SKU: A308999
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1991] antibody to CRABP1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-CRABP1 Antibody [ARC1991]

Description

Rabbit monoclonal [ARC1991] antibody to CRABP1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 58-137 of human CRABP1 (P29762).

Sequence

TVRTTEINFKVGEGFEEETVDGRKCRSLATWENENKIHCTQTLLEGDGPKTYWTRELANDELILTFGADDVVCTRIYVRE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1991

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cellular retinoic acid-binding protein 1, Cellular retinoic acid-binding protein I, CRABP-I, RBP5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-CRABP1 Antibody [ARC1991] (A308999)

Western blot analysis of extracts of various cell lines, using Anti-CRABP1 Antibody [ARC1991] (A308999) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-CRABP1 Antibody [ARC1991] (A308999)

Western blot analysis of extracts of Mouse testis, using Anti-CRABP1 Antibody [ARC1991] (A308999) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-CRABP1 Antibody [ARC1991] (A308999)

Immunohistochemistry analysis of paraffin-embedded rat cornea using Anti-CRABP1 Antibody [ARC1991] (A308999) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-CRABP1 Antibody [ARC1991] (A308999)

Immunohistochemistry analysis of paraffin-embedded rat retina using Anti-CRABP1 Antibody [ARC1991] (A308999) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.