Anti-COX IV Anticorps (A304966)

Name: Anti-COX IV Antibody
Brand: Antibodies.com
SKU: A304966
Price: 500.0 USD
Availability: InStock

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$500

Anticorps polyclonal de lapin contre COX IV pour WB et ICC/IF.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

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Mots clés :

Mitochondrial Loading Control Mitochondrial Marker

Spécifications

Nom du produit

Anti-COX IV Antibody

Description du produit

Rabbit polyclonal antibody to COX IV.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivité

Human, Mouse, Rat

Immunogène

Synthetic peptide of Human COX-4.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Masse moléculaire

~16 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AL024441, COX 4, COX IV 1, COX IV-1, Cox4, COX4-1, COX41_HUMAN, Cox4a, COX4B, COX4I1, COX4I2, COX4L2, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome C Oxidase subunit IV, Cytochrome c oxidase subunit IV isoform 1, Cytochrome c oxidase subunit IV isoform 2 (lung), Cytochrome c oxydase subunit 4, dJ857M17.2, MGC105470, MGC72016, mitochondrial

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-COX IV Antibody (A304966)

Western blot analysis of human HeLa and HEK293T cell lysates showing detection of 19.5 kDa COX-4 protein using Anti-COX IV Antibody (A304966) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 19.5 kDa.

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Figure 2: Immunofluorescence - Anti-COX IV Antibody (A304966)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-COX IV Antibody (A304966), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Mitochondrion. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) COX-4 Antibody (D) Composite.

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Figure 3: Immunofluorescence - Anti-COX IV Antibody (A304966)

Immunocytochemistry/Immunofluorescence analysis of mouse NIH 3T3 (mouse fibroblast cell line), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-COX IV Antibody (A304966), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Mitochondrion Inner Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) COX-4 Antibody (D) Composite.