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Anti-CoREST Anticorps [ARC2044] (A307585)

Nom du produit
Anti-CoREST Antibody [ARC2044]
Description du produit
Rabbit monoclonal [ARC2044] antibody to CoREST.
Applications
WB, IHC, IP, ChIP
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CoREST/RCOR1 (Q9UKL0).
Séquence
MPAMVEKGPEVSGKRRGRNNAAASASAAAASAAASAACASPAATAASGAAASSASAAAASAAAAPNNGQNKSLAAAAPNGNSSSNSWEEGSSGSSSDEEH
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC2044
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
65 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CoREST Antibody [ARC2044] (A307585) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CoREST Antibody [ARC2044] (A307585)
Western blot analysis of extracts of various cell lines, using Anti-CoREST Antibody [ARC2044] (A307585) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Western Blot - Anti-CoREST Antibody [ARC2044] (A307585) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-CoREST Antibody [ARC2044] (A307585)
Western blot analysis of extracts of C2C12 cells, using Anti-CoREST Antibody [ARC2044] (A307585) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry - Anti-CoREST Antibody [ARC2044] (A307585) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-CoREST Antibody [ARC2044] (A307585)
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-CoREST Antibody [ARC2044] (A307585) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CoREST Antibody [ARC2044] (A307585) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-CoREST Antibody [ARC2044] (A307585)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-CoREST Antibody [ARC2044] (A307585) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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