COMT expression in Human Testes lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-COMT Antibody (A83361) at 0.3µg/ml and detected by chemiluminescence.
COMT expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in Tris/EDTA buffer, pH 9. Staining was performed with Anti-COMT Antibody (A83361) at 4µg/ml and revealed with horseradish peroxidase (HRP).
COMT expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-COMT Antibody (A83361) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
COMT expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-COMT Antibody (A83361) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
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