Rabbit polyclonal antibody to Clostridium difficile Toxin A.
Specificity
This antibody recognises Clostridium difficile toxin A. Clostridium difficile is a gram-positive motile bacterium and the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density, and the administration of antibiotics.Toxin A (TcdA) is a large exotoxin which is an essential virulence factor for C. difficile infection. TcdA has a molecular mass of ~308 kDa and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways.
Applications
ELISA
Reactivity
Bacterial
Immunogen
Synthetic peptide sequence CTIDGKKYYFN corresponding to amino acids 2123 - 2132 of the native Clostridium difficile Toxin A conjugated to Keyhole Limpet Haemocyanin.
Sequence
CTIDGKKYYFN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein G affinity chromatography.
Concentration
5 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Clostridium difficile toxin A antibody recognizes Clostridium difficile toxin A. Clostridium difficile is a gram-positive motile bacterium and the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density, and the administration of antibiotics.Toxin A (TcdA) is a large exotoxin which is an essential virulence factor for C. difficile infection. TcdA has a molecular mass of ~308 kDa and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways.
