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Anti-CLC3 Anticorps [S258-5] (A304752)

Immunocytochemistry/Immunofluorescence - Anti-CLC3 Antibody [S258-5] (A304752) - Antibodies.com Western Blot - Anti-CLC3 Antibody [S258-5] (A304752) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-CLC3 Antibody [S258-5] (A304752) - Antibodies.com
Anticorps Validé par Knockout
$500
Product Datasheet

Anticorps monoclonal [S258-5] de souris contre CLC3 pour WB, IHC et ICC/IF.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-CLC3 Antibody [S258-5]
Description du produit
Mouse monoclonal [S258-5] antibody to CLC3.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100
Reactivité
Human, Mouse, Rat
Réactivité croisée
This antibody does not cross-react with CLCN4 or CLCN5 - based on KO validation data.
Immunogène
Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3.
Hôte
Mouse
Clonalité
Monoclonal
Clone
S258-5
Isotype
IgG1
Conjuguer

Unconjugated

Purification
Protein G purification.
Concentration
1 mg/ml
Masse moléculaire
~90 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Chloride channel 3, Chloride channel protein 3, Chloride channel, voltage-sensitive 3, Chloride transporter ClC 3, Chloride transporter ClC-3, Chloride transporter ClC3, chloride voltage-gated channel 3, ClC 3, ClC-3, CLCN 3, CLCN3, CLCN3_HUMAN, DKFZp564I0463, H(+)/Cl(-) exchange transporter 3
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Immunocytochemistry/Immunofluorescence - Anti-CLC3 Antibody [S258-5] (A304752) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Immunofluorescence - Anti-CLC3 Antibody [S258-5] (A304752)
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-CLC3 Antibody [S258-5] (A304752), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Clcn3 Antibody (D) Composite.
Western Blot - Anti-CLC3 Antibody [S258-5] (A304752) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-CLC3 Antibody [S258-5] (A304752)
Western blot analysis of rat brain membrane showing detection of ~90 kDa CIcn3 protein using Anti-CLC3 Antibody [S258-5] (A304752) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~90 kDa.
Immunocytochemistry/Immunofluorescence - Anti-CLC3 Antibody [S258-5] (A304752) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-CLC3 Antibody [S258-5] (A304752)
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-CLC3 Antibody [S258-5] (A304752), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Clcn3 Antibody. (D) Composite.
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