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CKS1 Cell Based Kit Elisa (A103545)

Nom du produit
CKS1 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This CKS1 Cell Based ELISA Kit allows for the detection of CKS1 and the effects that certain stimulation conditions have on CKS1 expression in different cell lines. Qualitative determination of CKS1 concentration is achieved by an indirect ELISA format. In essence, the CKS1 is captured by Anti-CKS1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CKS1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
CDC2 associated protein CKS1, CDC28 protein kinase 1, CDC28 protein kinase 1B, CDC28 protein kinase regulatory subunit 1B, Cell division control protein CKS1, CKS 1, CKS-1, Cks1b, CKS1_HUMAN, Ckshs1, Cyclin dependent kinases regulatory subunit 1, Cyclin-dependent kinases regulatory subunit 1, NB4 apoptosis/differentiation related protein, PNAS 143, PNAS 16, PNAS 18
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - CKS1 Cell Based ELISA Kit (CB5933)
Western blot analysis of extracts from Jurkat cells, treated with serum 20% 15’, using Anti-CKS1 Antibody.
Protocol Diagram - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: CKS1 Cell Based ELISA Kit (CB5933)
A diagram showing the protocol for CKS1 Cell Based ELISA Kit.
Protocol Summary - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: CKS1 Cell Based ELISA Kit (CB5933)
A summary of the protocol for CKS1 Cell Based ELISA Kit.
Western Blot - Control Antibody - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - CKS1 Cell Based ELISA Kit (CB5933)
The mouse monoclonal antibody to GAPDH is used as a positive control inCKS1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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