Western Blot - Anti-Chk2 (phospho Ser516) Antibody (A11080)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Chk2 (phospho Ser516) Antibody (A11080) at 1:1,000 dilution or Chk2 antibody (A0466). NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.
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