Anti-Chk1 Antibody (A295417)

Name: Anti-Chk1 Antibody
Brand: Antibodies.com
SKU: A295417
Price: 490.0 USD
Availability: InStock

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$490

Rabbit polyclonal antibody to Chk1 for WB, IP and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-9 business days.

Specifications

Name

Anti-Chk1 Antibody

Description

Rabbit polyclonal antibody to Chk1.

Applications

WB, IP, IHC

Dilutions

IHC: 1:1,000-1:5,000, IP: 2-10 µg/1 mg lysate, WB: 1:1,000-1:5,000

Reactivity

Human

Immunogen

Synthetic peptide within amino acids 250-300 of human Chk1.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunohistochemistry - Anti-Chk1 Antibody (A295417)

Sample: FFPE section of human breast carcinoma. Antibody: Anti-Chk1 Antibody (A295417) was used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB.

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Immunoprecipitation - Anti-Chk1 Antibody (A295417)

Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Anti-Chk1 Antibody (A295417) was used for IP at 6 µg/mg lysate. Chk1 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated Chk1, Anti-Chk1 Antibody (A295417) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Western Blot - Anti-Chk1 Antibody (A295417)

Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TMCK cells. Antibodies: Anti-Chk1 Antibody (A295417) was used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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