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Anti-CHCHD2 Anticorps (A88474)

Nom du produit
Anti-CHCHD2 Antibody
Description du produit
Rabbit polyclonal antibody to CHCHD2.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1).
Séquence
TLGHAITGGFSGGSNAEPARPDITYQEPQGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCR
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
16 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
16.7kD protein, Aging Associated Gene 10 Protein, Aging-associated gene 10 protein, C7orf17, CHCH2_HUMAN, CHCHD 2, Coiled Coil Helix Coiled Coil Helix Domain Containing 2, Coiled coil helix coiled coil helix domain containing protein 2, mitochondrial, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial, HCV NS2 trans regulated protein, HCV NS2 trans-regulated protein, NS2TP
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CHCHD2 Antibody (A88474) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CHCHD2 Antibody (A88474)
Western blot analysis of extracts of various cell lines, using Anti-CHCHD2 Antibody (A88474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-CHCHD2 Antibody (A88474) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-CHCHD2 Antibody (A88474)
Western blot analysis of extracts from normal (control) and CHCHD2 knockout (KO) 293T cells, using Anti-CHCHD2 Antibody (A88474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-CHCHD2 Antibody (A88474) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-CHCHD2 Antibody (A88474)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-CHCHD2 Antibody (A88474) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CHCHD2 Antibody (A88474) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-CHCHD2 Antibody (A88474)
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-CHCHD2 Antibody (A88474) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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