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CEBPB Cell Based Kit Elisa (A103470)

Nom du produit
CEBPB Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This CEBPB Cell Based ELISA Kit allows for the detection of CEBPB and the effects that certain stimulation conditions have on CEBPB expression in different cell lines. Qualitative determination of CEBPB concentration is achieved by an indirect ELISA format. In essence, the CEBPB is captured by Anti-CEBPB Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CEBPB Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
AGP/EBP, C EBP beta, C/EBP beta, C/EBP related protein 2, CCAAT Enhancer Binding Protein beta, CCAAT/enhancer-binding protein beta, CEBP Beta, CEBPB_HUMAN, CRP2, IL 6DBP, IL6DBP, Interleukin 6 dependent binding protein, LAP, Liver activator protein, Liver enriched transcriptional activator, NF IL6, NFIL6, Nuclear factor NF IL6, Nuclear factor NF-IL6, SF B, SFB, Silencer factor B, TCF-5, TCF5, Transcription factor 5
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - CEBPB Cell Based ELISA Kit (CB5848) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - CEBPB Cell Based ELISA Kit (CB5848)
Western blot analysis of extracts from Jurkat cells, using Anti-CEBPB Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - CEBPB Cell Based ELISA Kit (CB5848) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: CEBPB Cell Based ELISA Kit (CB5848)
A diagram showing the protocol for CEBPB Cell Based ELISA Kit.
Protocol Summary - CEBPB Cell Based ELISA Kit (CB5848) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: CEBPB Cell Based ELISA Kit (CB5848)
A summary of the protocol for CEBPB Cell Based ELISA Kit.
Western Blot - Control Antibody - CEBPB Cell Based ELISA Kit (CB5848) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - CEBPB Cell Based ELISA Kit (CB5848)
The mouse monoclonal antibody to GAPDH is used as a positive control inCEBPB Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.

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