Figure 1: Western Blot - Anti-Cdk6 Antibody (A13498)
Western blot analysis of HeLa, using Anti-Cdk6 Antibody (A13498) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cdk6 Antibody (A13498) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 3: Western Blot - Anti-Cdk6 Antibody (A13498)
Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-Cdk6 Antibody (A13498). This Western blot was performed on the immunoprecipitate using Anti-Cdk6 Antibody (A13498) at a dilution of 1:1000.
Produits alternatifs à Anti-Cdk6 Anticorps (A13498)