Anti-CDK1 (phospho Tyr15) Anticorps (A16400)

Name: Anti-CDK1 (phospho Tyr15) Antibody
Brand: Antibodies.com
SKU: A16400
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre CDK1 (phospho Tyr15) pour WB, IHC, ICC/IF et IP.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Centrosome Marker

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Anti-CDK1 Anticorps [POH-1]

Anticorps monoclonal [POH-1] de souris contre CDK1 pour IP, WB, IHC-P et ICC.

Spécifications

Nom du produit

Anti-CDK1 (phospho Tyr15) Antibody

Description du produit

Rabbit polyclonal antibody to CDK1 (phospho Tyr15).

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around Y15 of human CDK1 (NP_001777.1).

Séquence

GTYGV

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

34 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Cdc 2, Cdc2, CDC28A, CDK 1, CDK1_HUMAN, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cell Divsion Cycle 2 Protein, Cyclin Dependent Kinase 1, Cyclin-dependent kinase 1, DKFZp686L20222, MGC111195, p34 Cdk1, p34 protein kinase, P34CDC2

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (8)

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Figure 1: Western Blot - Anti-CDK1 (phospho Tyr15) Antibody (A16400)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-CDK1 (phospho Tyr15) Antibody (A16400) at 1:1,000 dilution or CDK1 antibody (A0220). HeLa cellls were treated by nocodazole (50 ng/ml) at 37°C for 20 hours or Hydroxyurea (4 mM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-CDK1 (phospho Tyr15) Antibody (A16400)

Western blot analysis of extracts of various cell lines, using Anti-CDK1 (phospho Tyr15) Antibody (A16400) at 1:1,000 dilution. HeLa and NIH/3T3 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-CDK1 (phospho Tyr15) Antibody (A16400)

Western blot analysis of extracts of C6 cells, using Anti-CDK1 (phospho Tyr15) Antibody (A16400) at 1:1,000 dilution. C6 cells were treated by Nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 4: Immunohistochemistry - Anti-CDK1 (phospho Tyr15) Antibody (A16400)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-CDK1 (phospho Tyr15) Antibody (A16400) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.