Anti-CDK1 Antibody (A89294)

Name: Anti-CDK1 Antibody
Brand: Antibodies.com
SKU: A89294

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This product is discontinued

Anti-CDK1 Antibody (A89294) has been discontinued and is no longer available.

View all CDK1 Antibodies.

Specifications

Name

Anti-CDK1 Antibody

Description

Rabbit polyclonal antibody to CDK1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 208-258 of human CDK1 (NP_001777.1).

Sequence

SEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDC2, CDC28A, CDK10, CDKN1, Cell division control protein 2 homolog, Cell division protein kinase 1, Cell division protein kinase 10, Cyclin-dependent kinase 1, Cyclin-dependent kinase 10, p34 protein kinase, P34CDC2, Serine/threonine-protein kinase PISSLRE

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-CDK1 Antibody (A89294)

Western blot analysis of extracts of various cell lines, using Anti-CDK1 Antibody (A89294) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Immunohistochemistry - Anti-CDK1 Antibody (A89294)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-CDK1 Antibody (A89294) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-CDK1 Antibody (A89294)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-CDK1 Antibody (A89294) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.