Anti-CD99 Anticorps [DN16] (A280262)

Anti-CD99 Antibody [DN16]

Mouse monoclonal [DN16] antibody to CD99.

This antibody recognises human CD99, also known as E2 antigen, MIC2 or 12E7. CD99 is a 185 amino acid ~32 kDa single pass type I transmembrane O-glycosylated glycoprotein. Three isoforms can be producted by alternative splicing. Epitope analysis of the DN16 clone suggests the antibody recognises a minimal peptide sequence "LPDNENKK" located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II. CD99 expression is notable in the testis, pancreas, bone marrow, lymph nodes and spleen. CD99 is expressed on all classes of leukocytes and tends to be highest on immature cells.Functionally CD99 has been found to be involved in cellular adhesion/aggregation and apoptosis.

WB, Flow Cytometry, IHC-Fr

WB: 1:1,000, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human

Mouse

Monoclonal

DN16

IgG1

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti human CD99 antibody, clone DN16 recognizes human CD99, also known as E2 antigen, MIC2 or 12E7. CD99 is a 185 amino acid ~32 kDa single pass type I transmembrane O-glycosylated glycoprotein. Three isoforms can be producted by alternative splicing. Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence "LPDNENKK" located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gil et al. 2002).CD99 expression is notable in the testis, pancreas, bone marrow, lymph nodes and spleen. CD99 is expressed on all classes of leukocytes and tends to be highest on immature cells.Functionally CD99 has been found to be involved in cellular adhesion/aggregation (Krisanaprakornkit et al. 2013) and apoptosis (Sciandra et al. 2014).

12E7, Antigen identified by monoclonal 12E7, Y homolog, Antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigen, CD99 molecule, CD99_HUMAN, Cell surface antigen 12E7, Cell surface antigen HBA 71, Cell surface antigen O13, E2 antigen, HBA71, MIC 2X, MIC 2Y, MIC2, MIC2 (monoclonal, MIC2X, MIC2Y, MSK5X, Protein MIC2, Surface antigen MIC2, T cell surface glycoprotein E2, T-cell surface glycoprotein E2