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Anti-CD8 alpha Anticorps [ARC0329] (A306457)

Nom du produit
Anti-CD8 alpha Antibody [ARC0329]
Description du produit
Rabbit monoclonal [ARC0329] antibody to CD8 alpha.
Applications
WB, IHC, Flow Cytometry
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, Flow Cytometry: 1:50-1:200
Reactivité
Human
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 136-235 of human CD8A (P01732).
Séquence
KPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCNHRNRRRVCKCPRPVVKSGDKPSLSARYV
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0329
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
35 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
alpha polypeptide (p32), CD8, CD8 antigen alpha polypeptide, CD8 antigen alpha polypeptide (p32), CD8a, CD8A antigen, CD8A molecule, CD8A_HUMAN, Leu2, Leu2 T lymphocyte antigen, Ly3, LYT3, MAL, OKT8 T cell antigen, OTTHUMP00000160760, OTTHUMP00000160764, OTTHUMP00000203528, OTTHUMP00000203721, p32, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen, T8/Leu-2 T-lymphocyte differentiation antigen
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CD8 alpha Antibody [ARC0329] (A306457) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CD8 alpha Antibody [ARC0329] (A306457)
Western blot analysis of extracts of various cell lines, using Anti-CD8 alpha Antibody [ARC0329] (A306457) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-CD8 alpha Antibody [ARC0329] (A306457) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-CD8 alpha Antibody [ARC0329] (A306457)
Immunohistochemistry analysis of paraffin-embedded human intestinal T-cell lymphoma using Anti-CD8 alpha Antibody [ARC0329] (A306457) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD8 alpha Antibody [ARC0329] (A306457) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-CD8 alpha Antibody [ARC0329] (A306457)
Immunohistochemistry analysis of paraffin-embedded human T-cell malignant lymphoma using Anti-CD8 alpha Antibody [ARC0329] (A306457) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD8 alpha Antibody [ARC0329] (A306457) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-CD8 alpha Antibody [ARC0329] (A306457)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD8 alpha Antibody [ARC0329] (A306457) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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