This antibody recognises a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes.
Applications
IHC-P, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:50 - 1:100, IHC-Fr: 1:100 - 1:500, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
Purified rat spleen L-CA.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-33
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes (Barclay et al. 1987).