Anti-CD45RA Anticorps [111-1C5] (A249813)

Anti-CD45RA Antibody [111-1C5]

Mouse monoclonal [111-1C5] antibody to CD45RA.

This antibody recognizes a protein of 205kDa-220kDa, identified as CD45RA which is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

Stimulated human leukocytes.

Mouse

Monoclonal

111-1C5

IgG1

kappa

Protein A/G chromatography.

200 µg/ml

205-220 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD45RA Antibody [111-1C5] - BSA and Azide free (A252993).

B220, CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, L-CA, LCA, Leukocyte common antigen, Loc, Ly-5, LY5, Ly5, homolog of, Lymphocyte antigen 5, Lyt-4, Protein tyrosine phosphatase, receptor type C, Protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, RT7, T200, T200 glycoprotein, T200 leukocyte common antigen