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Anti-CD45 Anticorps (A88986)

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Flow Cytometry - Anti-CD45 Antibody [MEM-28] (A85888) - Antibodies.com
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Anti-CD45 Anticorps [2D1]
Anticorps monoclonal [2D1] de souris contre CD45 pour Flow Cytometry.
Nom du produit
Anti-CD45 Antibody
Description du produit
Rabbit polyclonal antibody to CD45.
Applications
WB, ICC/IF, IP
Recommander des dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 1210-1306 of human CD45 (NP_002829.3).
Séquence
GMVSTFEQYQFLYDVIASTYPAQNGQVKKNNHQEDKIEFDNEVDKVKQDANCVNPLGAPEKLPEAKEQAEGSEPTSGTEGPEHSVNGPASPALNQGS
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
200 - 250 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
B220, CD 45, CD45 antigen, CD45R, GP180, L-CA, LCA, Leukocyte common antigen, loc, Ly-5, LY5, Ly5, homolog of, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyrosine phosphatase receptor type c polypeptide, Protein tyrosine phosphatase, receptor type C, protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CD45 Antibody (A88986) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CD45 Antibody (A88986)
Western blot analysis of extracts of Jurkat cells, using Anti-CD45 Antibody (A88986) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-CD45 Antibody (A88986) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunofluorescence - Anti-CD45 Antibody (A88986)
Immunofluorescence analysis of Jurkat cells using Anti-CD45 Antibody (A88986) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD45 Antibody (A88986) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-CD45 Antibody (A88986)
Immunofluorescence analysis of rat spleen cells using Anti-CD45 Antibody (A88986) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD45 Antibody (A88986) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-CD45 Antibody (A88986)
Immunofluorescence analysis of mouse spleen cells using Anti-CD45 Antibody (A88986) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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