Anti-CD3D Antibody [ARC1741] (A308414)

Western blot analysis of extracts of Jurkat cells, using Anti-CD3D Antibody [ARC1741] (A308414) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of rat spleen using Anti-CD3D Antibody [ARC1741] (A308414) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of mouse spleen using Anti-CD3D Antibody [ARC1741] (A308414) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Flow cytometry analysis of Jurkat cells, stained with Rabbit IgG isotype control (2.5 µg/ml, blue line) or Anti-CD3D Antibody [ARC1741] (A308414), (2.5 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line).