Recombinant rabbit monoclonal [DM136] antibody to CD34.
Applications
ELISA, Flow Cytometry
Recommander des dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivité
Human
Hôte
Rabbit
Clonalité
Monoclonal
Clone
DM136
Isotype
IgG
Conjuguer
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Forme du produit
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Notes générales
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plate pre-coated by 1 µg/ml (100 µl/well) Recombinant Human CD34 Protein (6×His Tag) (A318258) can bind Anti-CD34 Antibody [DM136] - Azide free (A318571) in a linear range of 0. 1-12 µg/ml.
Flow cytometry analysis with Anti-CD34 Antibody [DM136] - Azide free (A318571) on Expi293 cells transfected with human CD34 (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
Produits alternatifs à Anti-CD34 Anticorps [DM136] (A318571)