Mouse monoclonal [4H11[APG]] antibody to CD34 (PE-DyLight® 594).
Spécificité
This antibody reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells. It is gradually lost on progenitor cells. This antibody completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Applications
Flow Cytometry
Recommander des dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivité
Human
Immunogène
Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Hôte
Mouse
Clonalité
Monoclonal
Clone
4H11[APG
Isotype
IgG1
Conjuguer
PE-DyLight® 594
Purification
This antibody is conjugated with tandem dye PE-DyLight® 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
MW prédit
110-115 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Stockage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!