Anti-CD3 zeta (phospho Y142) Anticorps [EM-54] (A86525)

Name: Anti-CD3 zeta (phospho Y142) Antibody [EM-54]
Brand: Antibodies.com
SKU: A86525
Price: 195.0 USD
Availability: InStock

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$195

Anticorps monoclonal [EM-54] de souris contre CD3 zeta (phospho Y142) pour Flow Cytometry et WB.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Qualité 100 % Garantie

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Spécifications

Nom du produit

Anti-CD3 zeta (phospho Y142) Antibody [EM-54]

Description du produit

Mouse monoclonal [EM-54] antibody to CD3 zeta (phospho Y142).

Spécificité

This antibody recognizes phosphorylated intracellular tyrosine 142 of CD3 zeta chain, which is a component of TCR/CD3 complex expressed on T cells.

Applications

Flow Cytometry, WB

Recommander des dilutions

Flow Cytometry: 1-4 µg/ml; Intracellular staining.

Reactivité

Human, Mouse

Immunogène

CP1 (pTyr142) - KLH.

Hôte

Mouse

Clonalité

Monoclonal

Clone

EM-54

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

4930549J05Rik, A430104F18Rik, AW552088, CD247, CD247 antigen, Cd247 molecule, Cd3, CD3 antigen, zeta polypeptide, isoform CRA_b, CD3 antigen, zeta subunit, CD3-eta, CD3H, CD3Q, Cd3z, CD3Z antigen zeta polypeptide (TiT3 complex), CD3zeta, CD3zeta chain, CD3Z_HUMAN, MGC140430, T cell receptor T3 zeta chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3z, TCR zeta chain, TCRk, TCRZ, TCRzeta

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (1)

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Figure 1: Western Blot - Anti-CD3 zeta (phospho Y142) Antibody [EM-54] (A86525)

Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with Anti-CD3 zeta (Phospho-Tyr142) Antibody (A86525). 1: Wt mCD3zeta pervanadate non-stimulated. 2: Wt mCD3zeta pervanadate stimulated. 3: Mut. mCD3zeta Y/F2-6. 4: Mut. mCD3zeta Y/F1 and 3-6. 5: Mut. mCD3zeta Y/F1-2 and 4-6. 6: Mut. mCD3zeta Y/F1-3 and 5-6. 7: Mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native). 8: Mut. mCD3zeta Y/F1-5.