Anti-CD3 epsilon Anticorps [OKT3] (A281362)

Anti-CD3 epsilon Antibody [OKT3]

Mouse monoclonal [OKT3] antibody to CD3 epsilon.

This antibody recognises T-cell surface glycoprotein CD3 epsilon chain (CD3e), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3e is a subunit within the multiprotein cell surface receptor TCR-CD3 complex, which is expressed on the surface of mature T cells, NKT cells and thymocytes. The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell. Mice that lack the CD3e gene exhibit early arrest in T cell development and mutation of the gene in humans has been seen to result in severe combined immunodeficiency (SCID) (DeJarnette et al. 1998, de Saint Basile et al. 2004). Mouse anti Human CD3, clone OKT3 is commonly used in flow cytometry in the phenotyping human T cells. Characterization of the OKT3 clone has shown that it is able to compete and block the binding of the CD3 clones SK7 and UCHT1 to human T cells. The OKT3 clone has also been found to have immunosuppressive properties in vivo. As result of this, the clone was humanized to be used as a therapeutic drug called Muromonomab (Orthoclone). Muromonomab was used as an immunosuppressive including for after solid organ transplants, to prevent the activation of CD4- and CD8- T cells. However, the drug has since been discontinued due to the development of other treatments with similar efficacy and fewer side effects (Reichert 2012).

Flow Cytometry, Functional Assays, IHC-Fr

Human

Human peripheral blood lymphocytes.

Mouse

Monoclonal

OKT3

IgG2a

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD3 antibody, clone OKT3, recognizes T-cell surface glycoprotein CD3 epsilon chain (CD3e), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3e is a subunit within the multiprotein cell surface receptor TCR-CD3 complex, which is expressed on the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009). Mice that lack the CD3e gene exhibit early arrest in T cell development and mutation of the gene in humans has been seen to result in severe combined immunodeficiency (SCID) (DeJarnette et al. 1998, de Saint Basile et al. 2004). Mouse anti Human CD3, clone OKT3 is commonly used in flow cytometry in the phenotyping human T cells. Characterization of the OKT3 clone has shown that it is able to compete and block the binding of the CD3 clones SK7 and UCHT1 to human T cells (Li et al. 2005). The OKT3 clone has also been found to have immunosuppressive properties in vivo. As result of this, the clone was humanized to be used as a therapeutic drug called Muromonomab (Orthoclone). Muromonomab was used as an immunosuppressive including for after solid organ transplants, to prevent the activation of CD4- and CD8- T cells (Wilde & Goa 1996). However, the drug has since been discontinued due to the development of other treatments with similar efficacy and fewer side effects (Reichert 2012).

CD3e, CD3E antigen epsilon polypeptide, CD3e antigen epsilon polypeptide (TiT3 complex), CD3E antigen, epsilon subunit, CD3e molecule epsilon, CD3e molecule, epsilon (CD3 TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3E_HUMAN, IMD18, T cell antigen receptor complex epsilon subunit of T3, T cell surface antigen T3/Leu 4 epsilon chain, T cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE