Anti-CD3 epsilon Anticorps [APA1/1] (A85568)

Anticorps monoclonal [APA1/1] de souris contre CD3 epsilon pour Flow Cytometry, IP, WB, IHC-Fr et ICC.

Alternative Formats and ConjugatesAlternative Formats

Frais de livraison
Date de livraison
Livraison sous 4-6 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Produits recommandés pour Anti-CD3 epsilon Anticorps

Nom du produit
Anti-CD3 epsilon Antibody [APA1/1]
Description du produit
Mouse monoclonal [APA1/1] antibody to CD3 epsilon.
Spécificité
This antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Applications
Flow Cytometry, IP, WB, IHC-Fr, ICC
Recommander des dilutions
Flow Cytometry: Intracellular staining: 1-4 µg/ml; Positive Control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 µg/ml). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0, 1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 µg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS, IHC: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.
Reactivité
Mouse, Human
Immunogène
Purified human CD3 proteins isolated from thymus.
Hôte
Mouse
Clonalité
Monoclonal
Clone
APA1/1
Isotype
IgG1
Conjuguer

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonymes
CD3e, CD3E antigen epsilon polypeptide, CD3e antigen epsilon polypeptide (TiT3 complex), CD3E antigen, epsilon subunit, CD3e molecule epsilon, CD3e molecule, epsilon (CD3 TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3E_HUMAN, IMD18, T cell antigen receptor complex epsilon subunit of T3, T cell surface antigen T3/Leu 4 epsilon chain, T cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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