This antibody recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody has superior binding than the clone TB2.
Applications
Flow Cytometry, ICC
Dilutions
Flow Cytometry: 1-4 µg/ml.
Reactivity
Human
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
TB3
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunocytochemistry staining of human CD3 epsilon and CD3 gamma transfected COS cells using Anti-CD3 Antibody [UCHT1] (left) and Anti-CD3 Antibody [TB3] (right), indicated by Alexa Fluor 555 signal (red), and DNA stained by DAPI (blue).
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