Anti-CD3 Anticorps [KT3] (A280838)

Anti-CD3 Antibody [KT3]

Rat monoclonal [KT3] antibody to CD3.

This antibody recognises the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Mouse

CBAT6 thymocytes.

Rat

Monoclonal

KT3

IgG2a

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.

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