Anti-CD3 Antibody [CT-3] (A280150)

Anti-CD3 Antibody [CT-3]

Mouse monoclonal [CT-3] antibody to CD3.

This antibody recognises the chicken CD3 cell surface antigen expressed by T cells. The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.

Flow Cytometry, IHC-Fr, IP

Flow Cytometry: 1:50, Use 50µl of the suggested working dilution to label 10⁶ cells in 100µl

Chicken

Chicken thymocytes and Ig negative peripheral blood mononuclear cells.

Mouse

Monoclonal

CT-3

IgG1

500 µg/ml

Liquid

Supplied in Borate Buffered Saline.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.

CD3d, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain, T3D