Rabbit polyclonal antibody to CD28 (phospho Tyr218).
Spécificité
This antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
Applications
WB, ELISA
Recommander des dilutions
WB: 1:500-1:1000, ELISA: 1:40000
Reactivité
Human, Mouse
Immunogène
Synthetic peptide derived from human CD28 around the phosphorylation site of Tyr218 (amino acids 171-220).
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Masse moléculaire
25kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44
Figure 1: Western Blot - Anti-CD28 (phospho Tyr218) Antibody (A94934)
Western blot analysis of lysates from HepG2 cells treated with nocodazole 1µg/ml 16h using Anti-CD28 (phospho Tyr218) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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