This antibody recognises the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells. Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional assays it is recommended to use the low endotoxin and preservative free format of this antibody, MCA5779EL. Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4.
Applications
Flow Cytometry, Functional Assays
Recommander des dilutions
Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivité
Bovine
Immunogène
Plasmid DNA encoding the bovine CD28 gene.
Hôte
Mouse
Clonalité
Monoclonal
Clone
CC219
Isotype
IgG1
Conjuguer
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Stockage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
Notes générales
Mouse anti Bovine CD28, clone CC219, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells.Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional assays it is recommended to use the low endotoxin and preservative free format of this antibody, MCA5779EL.Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4 (Hogg et al. 2011).
Synonymes
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44
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