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Anti-CD28 Anticorps [ARC5082-01] (A309309)

Western Blot - Anti-CD28 Antibody [ARC5082-01] (A309309) - Antibodies.com Flow Cytometry - Anti-CD28 Antibody [ARC5082-01] (A309309) - Antibodies.com
$540
Product Datasheet

Anticorps monoclonal [ARC5082-01] de lapin contre CD28 pour WB et Flow Cytometry.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-CD28 Antibody [ARC5082-01]
Description du produit
Rabbit monoclonal [ARC5082-01] antibody to CD28.
Applications
WB, Flow Cytometry
Recommander des dilutions
WB: 1:500-1:1,000, Flow Cytometry: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant protein of human CD28.
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC5082-01
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
45 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CD28 Antibody [ARC5082-01] (A309309) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CD28 Antibody [ARC5082-01] (A309309)
Western blot analysis of extracts of various cell lines, using Anti-CD28 Antibody [ARC5082-01] (A309309) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Flow Cytometry - Anti-CD28 Antibody [ARC5082-01] (A309309) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Flow Cytometry - Anti-CD28 Antibody [ARC5082-01] (A309309)
Flow cytometric analysis of Anti-CD28 Antibody [ARC5082-01] (A309309) (10 µg/ml) in Jurkat cells (orange) compared to Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) Isotype control (blue) and non-staining control (red).

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