Recombinant Anti-CD27 Anticorps [DM57] (A318640)

Expi 293 cell line was transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Anti-CD27 Antibody [DM57] - BSA and Azide free (A318640) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).

Flow cytometry data of serially titrated Anti-CD27 Antibody [DM57] - BSA and Azide free (A318640) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit Anti-CD27 Monoclonal Antibody clones by titration of different concentrations onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of Anti-CD27 Antibody [DM57] - BSA and Azide free (A318640). (A) Anti-CD27 Antibody [DM57] - BSA and Azide free (A318640) does not bind to 293T cells that do not express CD27. (B) A clear peak shift of Anti-CD27 Antibody [DM57] - BSA and Azide free (A318640) was seen compared to the control when incubated with CD27-expressing Raji cells, indicating strong binding of Anti-CD27 Antibody [DM57] - BSA and Azide free (A318640) to CD27. Antibodies were incubated at 2 µg/ml.