Anti-CD204 Anticorps [2F8] (Biotin) (A279214)

Anti-CD204 Antibody [2F8] (Biotin)

Rat monoclonal [2F8] antibody to CD204 (Biotin).

This antibody recognises the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.

Flow Cytometry

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).

Mouse, Pig, Channel catfish

Raw 264 cell line.

Rat

Monoclonal

2F8

IgG2b

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Rat anti Mouse CD204 antibody, clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993). Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).

CD204 antigen, Macrophage acetylated LDL receptor I and II, Macrophage scavenger receptor 1, Macrophage Scavenger Receptor I, Macrophage scavenger receptor type III, Macrophage scavenger receptor types I and II, Msr 1, MSR1, MSRE_HUMAN, phSR1, phSR2, SCARA 1, SCARA1, Scavenger receptor class A member 1, Scavenger receptor class A, member 1, Scavenger receptor type A, Scvr, SR A, SRA

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.