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CD147 Cell Based Kit Elisa (A103144)

Nom du produit
CD147 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This CD147 Cell Based ELISA Kit allows for the detection of CD147 and the effects that certain stimulation conditions have on CD147 expression in different cell lines. Qualitative determination of CD147 concentration is achieved by an indirect ELISA format. In essence, the CD147 is captured by Anti-CD147 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CD147 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
5A11 antigen, 5F7, Basigin, Basigin (Ok blood group), BASI_HUMAN, Blood brain barrier HT7 antigen, Bsg, CD 147, CD147 antigen, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M 6, M6, M6 leukocyte activation antigen, Neurothelin, OK, OK blood group, OK blood group antigen, TCSF, Tumor cell derived collagenase stimulatory factor, Tumor cell-derived collagenase stimulatory factor
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - CD147 Cell Based ELISA Kit (CB5881) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - CD147 Cell Based ELISA Kit (CB5881)
Western blot analysis of extracts from RAW264.7 cells, using Anti-CD147 Antibody.
Protocol Diagram - CD147 Cell Based ELISA Kit (CB5881) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: CD147 Cell Based ELISA Kit (CB5881)
A diagram showing the protocol for CD147 Cell Based ELISA Kit.
Protocol Summary - CD147 Cell Based ELISA Kit (CB5881) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: CD147 Cell Based ELISA Kit (CB5881)
A summary of the protocol for CD147 Cell Based ELISA Kit.
Western Blot - Control Antibody - CD147 Cell Based ELISA Kit (CB5881) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - CD147 Cell Based ELISA Kit (CB5881)
The mouse monoclonal antibody to GAPDH is used as a positive control inCD147 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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