Mouse monoclonal [MEM-226] antibody to CD105 (PE-Cyanine 7).
Spécificité
This antibody reacts with an extracellular epitope of CD105, a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow, and erythroid precursors in fetal and adult bone marrow. It is also present on syncytiotrophoblast on placenta throughout pregnancy.
Applications
Flow Cytometry
Recommander des dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivité
Rat, Human
Immunogène
Recombinant Vaccinia virus containing the human CD105 (L isoform) cDNA.
Hôte
Mouse
Clonalité
Monoclonal
Clone
MEM-226
Isotype
IgG2a
Conjuguer
PE-Cyanine 7
Excitation: 565nm, Emission: 778nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
MW prédit
90 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Stockage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of HUVEC cell suspension stained with Anti-CD105 Antibody [MEM-226] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).
Separation of HUVEC cells (red-filled) from HEK-293 cells (black-dashed) in flow cytometry analysis using Anti-CD105 Antibody [MEM-226] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).