Anti-Caveolin-1 Anticorps (A82916)

Name: Anti-Caveolin-1 Antibody
Brand: Antibodies.com
SKU: A82916
Price: 480.0 USD
Availability: InStock

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$480

Anticorps polyclonal de chèvre contre Caveolin-1 pour ELISA, WB, IF, IHC et Flow Cytometry.

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Garantie de Correspondance des Prix

Alternative Formats and ConjugatesAlternative Formats

Biotin

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Caveolae Marker Endosomal Marker

Spécifications

Nom du produit

Anti-Caveolin-1 Antibody

Description du produit

Goat polyclonal antibody to Caveolin-1.

Applications

ELISA, WB, IF, IHC, Flow Cytometry

Recommander des dilutions

ELISA: 1:8,000, WB: 0.001-0.01µg/ml, IHC: 2.5µg/ml, IF: 10µg/ml

Reactivité

Human

Réactivité croisée

This antibody is not expected to cross react with Caveolin 2 and Caveolin 3

Immunogène

Synthetic peptide corresponding to the internal region of human Caveolin-1.

Séquence

C-DELSEKQVYDAH

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

22 kDa

MW prédit

20.5kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

BSCL3, CAV, CAV1, CAV1_HUMAN, caveolae protein, 22 kD, caveolin 1 alpha isoform, caveolin 1 beta isoform, Caveolin 1 caveolae protein 22kDa, Caveolin1, cell growth-inhibiting protein 32, CGL3, LCCNS, MSTP085, OTTHUMP00000025031, PPH3, VIP 21, VIP21

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (6)

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Figure 1: Western Blot - Anti-Caveolin-1 Antibody (A82916)

Caveolin-1 expression in A431 (A) and A549 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody was performed with Anti-Caveolin-1 Antibody (A82916) at 0.003µg/ml (A) or 0.001µg/ml (B) and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-Caveolin-1 Antibody (A82916)

Caveolin-1 expression in Human Heart lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Caveolin-1 Antibody (A82916) at 0.003µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-Caveolin-1 Antibody (A82916)

Caveolin-1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Caveolin-1 Antibody (A82916) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Golgi apparatus and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-Caveolin-1 Antibody (A82916)

Caveolin-1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Caveolin-1 Antibody (A82916) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.